biotinylated
Adjective
Meaning
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biotinylated (not comparable)
(biochemistry) Modified by reaction with a biotin group
Anagrams
Examples
Any other proteins binding to the biotinylated molecule will also stay with the bead and all other unbound proteins can be washed away.
Because both streptavidin and avidin bind biotin with high affinity (K d of 10 −14 mol/l to 10 −15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction.
The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest.
To circumvent this problem, beads conjugated to monomeric avidin can be used, which has a decreased biotin-binding affinity of ~10 −8 mol/l, allowing the biotinylated protein of interest to be eluted with excess free biotin.