crystal violet
Noun
Meaning
a green crystal (violet in water) used as a dye or stain or bactericide or fungicide or anthelmintic or burn treatment
Source: WordNetSynonyms
Hypernyms
Examples
Gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells making the cell wall more porous and incapable of retaining the crystal violet stain.
Iodide ( Imain or Imain ) interacts with CVmain and forms large complexes of crystal violet and iodine (CV–I) within the inner and outer layers of the cell.
DNA stained with crystal violet can be viewed under natural light without the use of a UV transilluminator which is an advantage, however it may not produce a strong band.
Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through a microscope.
Staining a cell with a dye such as Giemsa stain or crystal violet allows a microscopist to describe its size, shape, internal and external components and its associations with other cells.
The decolorization step is critical and must be timed correctly; the crystal violet stain is removed from both gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).