electroporation
Noun
Meaning
electroporation (countable and uncountable, plural electroporations)
(biotechnology) the creation of transient pores in the cell membrane, through the application of a high-voltage electrical pulse
Synonyms
Related terms
Examples
Although electroporation and dielectric breakdown both result from application of an electric field, the mechanisms involved are fundamentally different.
It is generally accepted that for a given pulse duration and shape, a specific transmembrane voltage threshold exists for the manifestation of the electroporation phenomenon (from 0.5 V to 1 V).
In contrast, during electroporation the lipid molecules are not chemically altered but simply shift position, opening up a pore which acts as the conductive pathway through the bilayer as it is filled with water.
Adherent cells can also be transfected using electroporation, providing researchers with an alternative to trypsinizing their cells prior to transfection.
Contrastingly, reversible electroporation occurs when the electricity applied with the electrodes is below the electric field threshold of the target tissue.
Electroporation is highly effective for transfecting cells in suspension using electroporation cuvettes.